Composite

Part:BBa_K3286216:Design

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-21)


rhamnose-inducible rhaBAD promoter + kaiABC Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 559
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1307
    Illegal BamHI site found at 2220
    Illegal XhoI site found at 2287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2943
    Illegal AgeI site found at 1039
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2350


Design Notes

No remarkable design consideration during the design of the sequence.


Source

This part comes from the genomic DNA of Synechococcus elongatus PCC 7942.

References